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Subsequently supplemental in vivo procedures have been introduced for probing the THC induced cannabimimetic results in animals. 1 peculiar product read more built use of a subset of white New Zealand rabbits that exhibited non-lethal convulsions when offered THC acutely plus the intensity with the convulsions diminished immediately after recurring THC administration, i.e., an indication of tolerance growth; when THC was discontinued, the animals regained their sensitivity to convulsion proneness (Consroe et al., 1982; Martin and Consroe, 1976). Combining steps of spontaneous locomotor action applying an open-area arena, rectal temperature recordings, analgesia assessment and indices of immobility (catalepsy) constitutes the cannabinoid tetrad bioassay (Martin et al., 1991). This has been and even now is Just about the most commonly applied bioassays for examining cannabimimetic action in rodents. Every of such four endpoints inside the tetrad by on their own is just not unique for cannabimimetic action and for that reason they have to be evaluated as being a cluster as opposed to independently to diminish the opportunity possibility for detecting Wrong positives (Wiley et al., 2006). This bioassay procedure has exhibited great correlation between cannabinoid receptor 1 (CB1R) binding affinity and potency (r = 0.

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